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codon optimized cptrxr open reading frames  (GenScript corporation)

 
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    GenScript corporation codon optimized cptrxr open reading frames
    Codon Optimized Cptrxr Open Reading Frames, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codon-optimized+open+reading+frames/codon+optimized+open+reading+frames/pm40304242-59-1-13
    Average 90 stars, based on 1 article reviews
    codon optimized cptrxr open reading frames - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: DES2 is a fatty acid Δ11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis
    Article Snippet: .. The open reading frames of DES1 and DES2 were codon‐optimized for expression in S. cerevisiae by Genscript, synthesized and supplied in the pUC‐57 vector. .. DES1 and DES2 were then excised using BamHI and SalI restriction sites and ligated into pHEY1 , for expression under the constitutive translational elongation factor EF‐1α ( TEF1 ) promoter. pHEY‐DES1, pHEY‐DES2 and pHEY‐EVC (empty vector) were transformed into S. cerevisiae wild‐type (WT) strain DTY‐11a ( MATa , leu2‐3 , leu2‐12 , trp1–1 , can1–100 , ura3–1 , ade2–1 ) and ole1Δ knockout strain AMY‐3α ( MATα , ole1Δ::LEU2 , trp1–1 , can1–100 , ura3–1 , ade2–1 ) , and colonies were selected on synthetic dextrose (SD) minimal medium agar plates lacking uracil.

    Synthesized:

    Article Title: DES2 is a fatty acid Δ11 desaturase capable of synthesizing palmitvaccenic acid in the arbuscular mycorrhizal fungus Rhizophagus irregularis
    Article Snippet: .. The open reading frames of DES1 and DES2 were codon‐optimized for expression in S. cerevisiae by Genscript, synthesized and supplied in the pUC‐57 vector. .. DES1 and DES2 were then excised using BamHI and SalI restriction sites and ligated into pHEY1 , for expression under the constitutive translational elongation factor EF‐1α ( TEF1 ) promoter. pHEY‐DES1, pHEY‐DES2 and pHEY‐EVC (empty vector) were transformed into S. cerevisiae wild‐type (WT) strain DTY‐11a ( MATa , leu2‐3 , leu2‐12 , trp1–1 , can1–100 , ura3–1 , ade2–1 ) and ole1Δ knockout strain AMY‐3α ( MATα , ole1Δ::LEU2 , trp1–1 , can1–100 , ura3–1 , ade2–1 ) , and colonies were selected on synthetic dextrose (SD) minimal medium agar plates lacking uracil.

    other:

    Article Title: Evaluation of a minimal array of Treponema pallidum antigens as biomarkers for syphilis diagnosis, infection staging, and response to treatment
    Article Snippet: Coding sequences (synthesized by GenScript as codon-optimized open reading frames for expression in E. coli ) were obtained based on the Nichols strain genome of T. pallidum ( ) (NC_021490.2 /CP004010.2) and were cloned either into the pEXP-5-NT (Life Technologies, Carlsbad, CA) plasmid, which adds an amino-terminal His-tag, or the pET28a+/pET23b+ vectors (Millipore Sigma, St. Louis, MO) between restriction sites that would retain the vector-encoded amino (pET28a+) or carboxyl-terminal (pET23b+) His-tag, respectively.

    Article Title: Evaluation of a minimal array of Treponema pallidum antigens as biomarkers for syphilis diagnosis, infection staging, and response to treatment.
    Article Snippet: Coding sequences (synthesized by GenScript as codon-optimized open reading frames for expression in E. coli) were obtained based on the Nichols strain genome of T. pallidum (32) (NC_021490.2 /CP004010.2) and were cloned either into the pEXP-5-NT (Life Technologies, Carlsbad, CA) plasmid, which adds an amino-terminal His-tag, or the pET28a+/pET23b+ vectors (Millipore Sigma, St. Louis, MO) between restriction sites that would retain the vector-encoded amino (pET28a+) or carboxyl-terminal (pET23b+) His-tag, respectively.

    Clone Assay:

    Article Title: Substrate Affinity Versus Catalytic Efficiency: Ancestral Sequence Reconstruction of tRNA Nucleotidyltransferases Solves an Enzyme Puzzle
    Article Snippet: .. The codon-optimized open reading frames of the four reconstructed enzyme candidates were ordered at GenScript (Rijswijk, Netherlands), cloned and recombinantly expressed in E. coli BL21 (DE3) Δ cca . .. The codon-optimized open reading frames of the four reconstructed enzyme candidates were ordered at GenScript (Rijswijk, Netherlands), cloned and recombinantly expressed in E. coli BL21 (DE3) Δ cca .



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